N. Verbruggen, M. Van Montagu, E. Messens
Aug 24, 1992
Citations
0
Influential Citations
9
Citations
Journal
FEBS Letters
Abstract
Azetidine‐2‐carboxylic acid, the 4‐membered ring noranalogue or proline, is regularly used in the study or proline metabolism as well as the study of protein conformation. We prepared ??‐[2,3‐3H]azetidine‐2‐carboxylic acid with an optimized 10% yield from commercially available 4‐amino‐[2,3‐3H]butyric acid. Purification was performed by fast‐protein liquid chromatography. The biological activity was checked in both Arabidopsis thaliana and Escherichia coli. The obtained specific activity or 10 mCl/mmol was sufficient for most uptake and incorporation studies.