W. Rui
2010
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Journal
Chemical Reagents
Abstract
The synthesis of 2,3-bis(2-sulfonate-4-methylphenyl)-5-cyan-2H-tetrazolium monosodium inner salt,which was a new highly water-soluble tetrazolium salt,was reported.It was prepared by sulfonation of p-toluidine in the presence of superacidic catalyst TiO2/SO2-4,followed by diazotization to give the diazo salt of p-toluidine-o-sulfonic acid,which was then subjected to coupling with cyanoacetic acid followed by oxidation with chlorine.It was found that the new tetrazolium compound is a potential redox indicator which can be used in cell activity tests.