G. Kloster, P. Laufer
Nov 1, 1980
Citations
0
Influential Citations
16
Citations
Journal
Journal of Labelled Compounds and Radiopharmaceuticals
Abstract
D,L-3-[11C]-Alanine was prepared by methylation with 11CH3I of tert butyl 2-isocyanoacetate and subsequent hydrolysis. Enzymatic reaction using glutamate pyruvate transaminase and lactate dehydrogenase in a coupled reaction yields L-3-[11C]-lactic acid. The radiochemical yield is 45% for D,L-alanine and 26.5% for L-lactic acid at a specifie activity of 0.45 mCi/μmole. Synthesis time including purification by hplc is 23 min for D,L-3-[11c]-alanine and 45 min for L-3-[11C]-lactic acid, respectively.