N. Gross, M. Rabinowitz
Mar 25, 1969
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Journal
The Journal of biological chemistry
Abstract
Abstract Tritiated 5-bromo-2'-deoxyuridine was used as a precursor for the synthesis in vivo of nuclear and mitochondrial deoxyribonucleic acid in rat liver. Radioactivity was incorporated into a band of mitochondrial DNA which had a higher buoyant density than previously existing unlabeled DNA. The difference in density between labeled and unlabeled mitochondrial DNA bands doubled if the DNA was denatured after the introduction of strand breakages to allow complete separation of complementary strands. The result suggests that replication of mitochondrial DNA is by a semiconservative mechanism and that there is little, if any, repair of the DNA. Similar results leading to the same conclusions were obtained for nuclear DNA.