Pang Li-hua
2009
Citations
0
Influential Citations
0
Citations
Journal
Chemistry & Bioengineering
Abstract
A new bifunctional chelate intermediate of time-resolved fluorescence immunoassay 4-bromo-2,6-bis(3-(bromomethyl)-1H-pyrazol-1-yl)pyridine was prepared from 4-bromo-2,6-bis(3-methyl-1H-pyrazol-1-yl)pyridine by diazotizing reaction and brominating reaction.The structure of the product was determined by IR,1HNMR,GC-MS and elemental analysis.The reaction conditions and reaction mechanism were also researched.At the same time,the main outgrowth 4-bromo-2-(3-(bromomethyl)-1H-pyrazol-1-yl)-6-(3-methyl-1H-pyrazol-1-yl)pyridine was separated and its structure was determined by 1HNMR,GC-MS and elemental analysis as well.And the target product could also be prepared from it,so the yield was increased greatly.