S. Tokutake, S. Saito, N. Yamaji
Oct 1, 1992
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Journal
Analytical Sciences
Abstract
Eight novel 2-chloro-4-nitropheny] maltotetraosides modified at the 6^4- and/or 4^4-position were synthesized for human pancreatic and salivary a-amylase substrates. In these compounds, 2-chloro-4-nitrophenyl O -[4,6-di- O -( N -isopro-pyl)carbamoyl- α - d -glucopyranosyl]-(l→4)-bis[ O - α - d -glucopyranosyl-(1→4)]- β - d -glucopyranoside (9) was rapidly hydrolyzed by the two amylases at a limited position to produce 2-chloro-4-nitrophenyl β - d -glucopyranoside. Taking advantage of the properties, 9 was found to be a good substrate for a human a-amylase assay using only β -glucosidase as a coupled enzyme.