G. Losse, W. Naumann, A. Winkler
1994
Citations
0
Influential Citations
3
Citations
Journal
Journal Fur Praktische Chemie-chemiker-zeitung
Abstract
The solid-phase synthesis of the dodecaribonucleotide ACCACUAAAGCG is described using the tert. butyloxycarbonyl (Boc) as 2′-OH-protecting group. The synthesis was carried out on a filter disc support Whatman 3 MM by the phosphotriester method. Under these conditions, the 2′-OH-Boc-group proved to be absolutely stable. Moreover, it is quantitatively and selectively removable by 4n HCl/dioxane. Thus, the group renders to be an excellent persistent 2′-OH-protection in ribonucleotide synthesis.