D. Boger, W. Tse
Sep 1, 2001
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0
Influential Citations
82
Citations
Quality indicators
Journal
Bioorganic & medicinal chemistry
Abstract
The viability of using thiazole orange as an alternative to ethidium bromide in a fluorescent intercalator displacement (FID) assay is explored by profiling the DNA binding affinity and sequence selectivity of netropsin. Utilizing a library of hairpin deoxyoligonucleotides containing all possible four base-pair sequences, the method provides a high resolution profile of the DNA binding properties of small molecules in a high throughput format.