N. Narasimhachari, J. Plaut, K. Leiner
Feb 2, 1972
Citations
0
Influential Citations
7
Citations
Journal
Journal of chromatography
Abstract
Abstract Procedures for the separation and quantitative determination of 3,4-dimethoxyphenethylamine as the isothiocyanate derivative are given and the thin-layer chromatographic properties of 3,4-dimethoxyphenethylamine isothiocyanate are described. The sensitivity of this method for qualitative identification on thin-layer chromatography is 0.003 μg and for quantitative determination 0.01 μg. Recovery experiments were carried out with varying quantities of 3,4-dimethoxyphenethylamine added to 80% of a 24-h urine collection. Levels as low as 5 μg of 3,4-dimethoxyphenethylamine added to a urine sample yielded a distinct 3,4-dimethoxyphenethylamine isothiocyanate spot on thin-layer chromatography when 10% of an aliquot of the final concentrate was spotted.