I. Choma, C. Kowalski, R. Lodkowski
Jul 1, 2008
Citations
0
Influential Citations
21
Citations
Journal
Journal of Liquid Chromatography & Related Technologies
Abstract
Abstract Cephalosporins, relatively new antibiotics related to penicillins, are widely used in the treatment of both human and veterinary diseases because of their broad spectrum of antibacterial activity and good pharmacokinetic properties. Cefacetril, belonging to cephalosporins, is commonly used in treating mastitis in cows. In the present paper, cefacetril excretion with milk was examined by means of the TLC-DB and HPLC method. Thin-layer chromatography-direct bioautography is the technique which combines TLC with microbiological detection. Semi-quantitative determination of cefacetril in milk by TLC-DB was compared with quantitative HPLC analysis. An exponential relationship was proposed for calibration curves in bioautography.