K. Mopper, William L. Stahovec, L. Johnson
1983
Citations
0
Influential Citations
27
Citations
Journal
Journal of Chromatography A
Abstract
Abstract Dimedone (5,5-dimethyl-1,3-cyclohexanedione) is a highly specific and extremely sensitive reagent for the determination of aldehydes. We have adapted this reagent to the high-performance liquid chromatographic analysis of these compounds by precolumn derivatization. The reaction and chromatographic conditions were optimized. Using fluorimetric detection, the standard deviation at the 28 pmol level was about ±2% for most aldehydes. The detection limit was found to be about 30 fmol per injected aldehyde, but could be decreased by a factor of 100 by a single extraction step. Intercalibration with the standard 2,4-dinitrophenylhydrazine technique gave good agreement. Applications to various environmental and industrial samples are illustrated.