M. S. Masri, M. Friedman
Jan 15, 1982
Citations
1
Influential Citations
19
Citations
Journal
Biochemical and Biophysical Research Communications
Abstract
Abstract The SH group of 2-mercaptoethylpyridine adds to the double bond of dehydroalanine residues in alkali-treated casein and acetylated casein to form S-β-(2-pyridylethyl)-L-cysteine (2-PEC) side chains. The generated cysteine derivative can be assayed, after protein hydrolysis, by standard amino acid analysis techniques. The novel transformation may be useful for assessing chemical and biological functions of dehydroalanine residues in proteins.