F. F. Campo, M. Hernandez-Asensio, J. Ramírez
Apr 21, 1975
Citations
1
Influential Citations
21
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Quality indicators
Journal
Biochemical and biophysical research communications
Abstract
Abstract The initial rate of uptake of methyl α-D-glucopyranoside by Escherichia coli is inhibited by respiration. The inhibition is more pronounced in mutant strains which cannot use the energy-rich state of the membrane to form ATP because of a defective Ca2+, Mg2+-activated ATPase. In both mutant and normal strains, the inhibition of glucoside uptake is not accompanied by an increase of the ATP content of the cells and is abolished by carbonyl cyanide m -chlorophenylhydrazone, a drug which dissipates membrane energy. It appears, therefore, that the inhibitory effect of respiration is mediated by the energy-rich state of the membrane and that ATP does not participate in the inhibition.