C. W. Tabor, H. Tabor
Aug 25, 1966
Citations
3
Influential Citations
82
Citations
Quality indicators
Journal
The Journal of biological chemistry
Abstract
Abstract Uptake processes for 1,4-diaminobutane, spermidine, and spermine have been studied in Escherichia coli. The uptake systems can be divided into two components. One is a rapid adsorption that is not dependent on active metabolic processes, and can be observed at 0° as well as at 37°. The adsorbed amine can be largely removed by washing the bacteria with the nonradioactive amine or with salt-containing media. The amount of the adsorbed amine was less than 1% of the added amine for 1,4-diaminobutane, 3 to 5% for spermidine, and 7 to 15% for spermine. The second is a larger, time-dependent component which depends on metabolic processes, does not occur at 0°, and is markedly decreased by the omission of glucose or by the addition of dinitrophenol. The accumulated amines cannot be removed by washing the bacteria with solutions of the nonradioactive amines; they are released, however, by treatment of the bacteria with toluene or butanol-1. Studies are also reported on the effect of pH, concentration, and inhibitors on these systems.