Neil Harper, B. Moore, P. Halling
May 29, 2000
Citations
0
Influential Citations
13
Citations
Journal
Tetrahedron Letters
Abstract
Abstract Mixtures of commercially available tridodecylamine and its hydrochloride dissolved in hexane or toluene have been used as acid-base buffers for a suspended immobilised enzyme. The activity of subtilisin Carlsberg increases with the proportion of free amine, to a similar extent in both solvents. This indicates that the buffer fixes the ionisation state of the enzyme in the range appropriate for catalytic activity.