D. McCann, T. Su
Jul 20, 1992
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Quality indicators
Journal
Neuroscience Letters
Abstract
Abstract Tris-HCl is the most commonly used buffer in studies of radioligand binding to σ receptors, with concentrations as high as 50 or 100 mM often used. We report here that these concentrations of Tris substantially inhibit (+)-[ 3 H]SKF-10,047 binding to σ receptors. The well-established inhibitory effect of Tris-HCl on ligand binding to PCP receptors did not contribute to the presently reported inhibition of (+)-[ 3 H]SKF-10,047 binding. The IC 50 of Tris, determined in the presence of 10 mM potassium phosphate buffer, was 15.4±1.2 mM ( n =3, pH 8.0, 25°C, 1 nM radioligand). Equilibrium saturation studies revealed an apparent competitive inhibition of binding.