S. Byun, H. Pedersen, C. Chin
1990
Citations
1
Influential Citations
36
Citations
Journal
Phytochemistry
Abstract
Abstract Accumulation of the benzophenanthridine alkaloids sanguinarine, chelerythrine, chelirubine and macarpine occurred in suspension cultures of Eschscholtzia californica . An enhanced production of some of these alkaloids was found in a two-phase culture system and in a system with elicitation in conjunction with two-phase culture. A versatile and practical accumulation phase for culture of E. californica was discovered. This phase was composed of silicone fluid, which accumulated a large amount of the alkaloid and greatly increased alkaloid production compared to cultures grown in its absence. Furthermore, elicitation in combination with two-phase culture additionally increased alkaloid production 10-fold.