Partha S. Mukherjee, H. Karnes
Sep 1, 1996
Citations
0
Influential Citations
38
Citations
Quality indicators
Journal
Biomedical chromatography : BMC
Abstract
Pre- and postcolumn derivatization with a suitable chromophore or fluorophore have often been utilized to enhance the sensitivity and selectivity of detection for analytes possessing a carboxyl group. The major classes of UV and fluorescent derivatization reagents include the coumarin analogues, alkyl halides, diazoalkanes, and amines. The derivatization reaction conditions, HPLC systems for separation of the conjugates and detection limits of various analytes are presented in this review. High mass sensitivity for various carboxylic acids have been achieved with the hydrazides, anthracenes, and diazoalkanes.