Mikiei Tanaka, Y. Mukohata, S. Yuasa
Sep 1, 1996
Citations
0
Influential Citations
4
Citations
Journal
Canadian Journal of Microbiology
Abstract
Using the extreme halophile Halobacterium halobium VO107, the utilization of D-leucine was studied in comparison with that of L-leucine. The initial uptake of D-leucine into cells was found to be less efficient than that of L-leucine, but like L-leucine, the D-leucine uptake was inhibited by uncouplers and an ATPase inhibitor, suggesting that an energy-dependent process was involved in its uptake. Following uptake, D-leucine was isomerized to L-leucine for further metabolism by an enzyme(s) that was induced in the presence of D-leucine in medium.Key words: Halobacterium halobium, archaebacteria, D-amino acid utilization, isomerization.