Laura Rubió, R. Solà, M. Romero
Jul 30, 2021
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Influential Citations
2
Citations
Quality indicators
Journal
Molecular nutrition & food research
Abstract
SCOPE It has been reported that the phenolic metabolite 3"-methoxycinnamic acid-4"-sulfate generated from 5-O-caffeoylquinic acid might have potential benefits in human health. However, the variation in 3"- and 4"-methylation of 3",4"-dihydroxycinnamic acid and its impact on the yield of this sulfate metabolite is unclear and has been poorly studied. METHODS AND RESULTS To address this aim, the excreted 3"-methoxy and 4"-methoxy metabolites in urine samples (24-h) were determined in 14 volunteers after an acute intake of 80 mg of red-fleshed apple (RFA) or white-fleshed apple (WFA). These methoxy metabolites were also determined in the same volunteers in a second acute intake after a six-week sustained consumption of the same products. CONCLUSION Seven 3"-methoxy and seven 4"-methoxy metabolites were determined, i.e., the free cinnamic and corresponding phenylpropanoic acid, plus their sulfate, glucuronide and glycine conjugates. In only six volunteers, five females and one male, was 4"-methylation preferred over 3"-methylation, but it was observed that an individual's 3"- : 4"-methylation ratio can change over time, and that the yield of 3"-methoxycinnamic acid-4"-sulfate was extremely variable, ranging from undetectable to 71% of the total C6 -C3 metabolites excreted, and any benefit accruing from this metabolite will not necessarily be available to all consumers. This article is protected by copyright. All rights reserved.