W. Sam, R. SudhakaranNairC, R. Ramachandran
Mar 25, 2017
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Abstract
Marine sponges belonging to the phylum porifera have been subject to anti-cancer studies which have yielded anticancer agents like spongothymidine and spongouridine. The present work is the in vitro pharmacological evaluation of the extract of the keratosid sponge Spongia officinalis var. Ceylonensis (Dendy) for anti-cancer activity. Purified fractions of Spongia officinalis induced anti-proliferative effects on A549, lung carcinoma cells with an IC 50 values of 1.24μg/μl, whereas it was relatively nontoxic to normal cell lines L929 at the mentioned concentrations. Flow cytometric analysis depicts inhibition at G2/M phase which can be considered significant. Overall results suggests potent anticancer effect of the extract which can find applications in therapeutic interventions. The flow cytometric analysis was performed using the MUSE cell cycle kit on MuseTM flow cytometer (Millipore, USA). The extract showed cytotoxicity on lung cancer cells by causing cell cycle arrest at G2/M phase.