Shujie Ma, Jia-Huan Liu, Xiaopeng Lu
Nov 13, 2019
Citations
0
Influential Citations
8
Citations
Quality indicators
Journal
Journal of agricultural and food chemistry
Abstract
Although action site of wilforine located in the muscle tissue of insects, the insecticidal mechanism of wilforine is not yet clear. This research explored the effects of wilforine on calcium signaling pathway using calcium imaging technique to reveal the insecticidal mechanism. It was confirmed that wilforine had strong cytotoxicity to Mythimna Separata myocytes with the IC50 values of 25.14 and 19.65 mg/L using CCK-8 and MTT methods, respectively. The morphological development of M. separate myocytes was also affected. Calcium imaging technique showed that intracellular calcium ion concentration ([Ca2+]i) was increased by 23.45% of the initial value after treated with 100 nM wilforine. However, wilforine did not increase [Ca2+]i after the myocytes pre-incubated by thapsigargin, and the [Ca2+]i could not be decreased by 50 μM ryanodine after treated with 100 nM wilforine. These results indicated that the targets of wilforine located in sarcoplasmic reticulum, and ryanodine receptor (RyR) is an important action target of wilforine. Furthermore, wilforine can also activate inositol triphosphate receptor (IP3R), which was confirmed through the use of 2-aminoethyl diphenylborinate (2-APB), an inhibitor of IP3R. Connected with previous researches, it can be concluded that wilforine affects the calcium signaling pathway by combining with RyR and IP3R, causing calcium dyshomeostasis, which result in insect paralysis and death.